链亲和素

[CAS号 ] 9013-20-1  
[分子式] C14H17BrClNO2S
[分子量] 378.709
[外观]白色粉末
[溶解度]溶于水
关于链霉亲和素
Streptavidin is a 52.8 kDa protein purified from the bacterium Streptomyces avidinii.链霉亲和素是从抗生物素蛋白链霉菌（Streptomyces avidinii）纯化的7 kDa蛋白。 Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B10 or vitamin H).链霉亲和素同四聚体对生物素（也称为维生素B14或维生素H）具有极高的亲和力。 With a dissociation constant (Kd) on the order of ≈XNUMX−XNUMX mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature.具有约XNUMX-XNUMX mol / L的解离常数（Kd），生物素与链霉亲和素的结合是自然界中最强的非共价相互作用之一。 Streptavidin is used extensively in molecular biology and bionanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (eg guanidinium chloride), detergents (eg SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.由于链霉亲和素-生物素复合物对有机溶剂，变性剂（如氯化胍），去污剂（如SDS，Triton），蛋白水解酶以及极端的温度和pH值具有抵抗力，因此链霉亲和素已广泛用于分子生物学和生物纳米技术。